Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)
![Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)](https://www.abcam.com/ps/products/239/ab239878/Images/ab239878-406356-anti-phospholipase-c-gamma-1-plc-gamma-1-antibody-ep1898-7y-bsa-and-azide-free-western-blot.jpg "Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1898-7Y] to Phospholipase C gamma 1/PLC-gamma-1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit monoclonal [EP1898-7Y] to Phospholipase C gamma 1/PLC-gamma-1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab239878 is the carrier-free version of ab76155. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab239878 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1898-7Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)
This data was developed using ab76155, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: hospholipase C gamma 1/PLC-gamma-1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Mouse brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76155 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76155 was shown to specifically react with Phospholipase C gamma 1/PLC-gamma-1 when Phospholipase C gamma 1/PLC-gamma-1 knockout samples were used. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab76155 at a dilution of 1/5000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)](https://www.abcam.com/ps/products/239/ab239878/Images/ab239878-397949-anti-phospholipase-c-gamma-1-plc-gamma-1-antibody-ep1898-7y-bsa-and-azide-free-western-blot.gif)
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155) at 1/10000 dilution + Jurkat cell lysates at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaThis data was developed using ab76155, the same antibody clone in a different buffer formulation.
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![Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)](https://www.abcam.com/ps/products/239/ab239878/Images/ab239878-4-benefits-of-recombinant-antibodies.png)
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)
