Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1898-7Y] to Phospholipase C gamma 1/PLC-gamma-1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies

  • Description

    Rabbit monoclonal [EP1898-7Y] to Phospholipase C gamma 1/PLC-gamma-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab239878 is the carrier-free version of ab76155. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239878 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)

    This data was developed using ab76155, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: hospholipase C gamma 1/PLC-gamma-1 knockout HAP1 cell lysate (20 µg)

    Lane 3: HepG2 cell lysate (20 µg)

    Lane 4: Mouse brain tissue lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab76155 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

     

    ab76155 was shown to specifically react with Phospholipase C gamma 1/PLC-gamma-1 when Phospholipase C gamma 1/PLC-gamma-1 knockout samples were used. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab76155 at a dilution of 1/5000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)
    Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155) at 1/10000 dilution + Jurkat cell lysates at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 149 kDa
    Observed band size: 149 kDa



    This data was developed using ab76155, the same antibody clone in a different buffer formulation.

     

  • Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)
    Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] - BSA and Azide free (ab239878)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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