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D-Mannitol Assay Kit (Colorimetric) (ab155890)

D-Mannitol Assay Kit (Colorimetric) (ab155890)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 20 min
  • Sensitivity: 10 µM

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Overview

  • Product name

    D-Mannitol Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Assay type

    Quantitative
  • Sensitivity

  • Range

    0 nmol/well - 20 nmol/well
  • Assay time

    0h 20m
  • Species reactivity

    Reacts with: Plants, Mammals, Fungi, Other species, Bacteria
  • Product overview

    In Abcam's D-Mannitol Assay Kit (Colorimetric) (ab155890), D-mannitol is converted to D-fructose by mannitol dehydrogenase in the presence of NAD to form NADH, which reduces a colorless probe to a chromogen with strong absorbance at 450 nm. This kit can also be used to measure L-arabitol, another sugar alcohol, as L-arabitol acts as substrate for mannitol dehydrogenase. The D-mannitol assay kit is fast (~20 minutes), sensitive and easy to use. This assay kit can detect mannitol level less than 1 nmol/reaction (

  • Notes

    D-Mannitol is one of the most abundant sugar alcohols in nature. It can be produced in the biosphere by a range of organisms such as bacteria, yeast, fungi and almost all plants. Mannitol functions as a carbohydrate storage depot, as a scavenger for reactive oxygen species and as an osmoprotectant. Recent studies have shown that increased mannitol content in plants protects them against fungal infections and improves resistance to drought and high salinity. The mannitol pathway reversibly converts fructose to mannitol and serves as the main carbohydrate cycle for fungi survival. Measurement of mannitol level is important for evaluating mannitol metabolic pathways and commercially, in developing fungi and drought resistant plants.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Mannitol Assay Buffer WM 1 x 27ml
    Mannitol Enzyme Mix Green 1 x 30µl
    Mannitol Standard Yellow 1 vial
    Mannitol Substrate Mix Red 1 vial
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Sugar Assays

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Mannitol Assay Buffer WM 1 x 27ml
    Mannitol Enzyme Mix Green 1 x 30µl
    Mannitol Standard Yellow 1 vial
    Mannitol Substrate Mix Red 1 vial
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Sugar Assays

Images

  • Functional Studies - D-Mannitol Assay Kit (Colorimetric) (ab155890)
    Functional Studies - D-Mannitol Assay Kit (Colorimetric) (ab155890)

    Mannitol measured in various samples showing quantity (nmol) per mg of extracted protein.

    Protein concentration for samples varied from 2 mg/mL to 7 mg/mL. Samples were diluted 2 fold.

  • Measurement of Mannitol in the extracts of Longyan & Lyche
    Measurement of Mannitol in the extracts of Longyan & Lyche

    Measurement of Mannitol in the extracts of Longyan & Lyche generated using ab155890

  • Mannitol Standard Curve
    Mannitol Standard Curve

    Mannitol Standard Curve generated using the method described in the protocol bookelt 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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