Anti-DNA PKcs antibody [18-2] (ab44815)
![Anti-DNA PKcs antibody [18-2] (ab44815)](https://www.abcam.com/ps/products/44/ab44815/Images/ab44815-268428-anti-dna-pkcs-antibody-18-2-western-blot.jpg "Anti-DNA PKcs antibody [18-2] (ab44815)")
Key features and details
- Mouse monoclonal [18-2] to DNA PKcs
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-DNA PKcs antibody [18-2]
See all DNA PKcs primary antibodies -
Description
Mouse monoclonal [18-2] to DNA PKcs -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Other Immunogen Type corresponding to Human DNA PKcs aa 1-2713. Immunogen is purified from HeLa cells.
Database link: P78527 -
Epitope
amino acids 1-2713 -
Positive control
- WB: Wildtype HAP1, SHSY5Y and K562 cell lysates. ICC/IF: Wildtype HAP1 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
18-2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-DNA PKcs antibody [18-2] (ab44815)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: DNA PKcs knockout HAP1 cell lysate (40 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: SHSY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab44815 observed at 460 kDa. Red - loading control, ab18251, observed at 52 kDa.ab44815 was shown to specifically react with DNA PKcs in iwld-type HAP1 cells. No band was observed when DNA PKcs knockout samples were examined. Wild-type and DNA PKcs knockout samples were subjected to SDS-PAGE. Ab44815 and ab18251 (loading control to alpha tubulin) were diluted at 1/200 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs antibody [18-2] (ab44815)](https://www.abcam.com/ps/products/44/ab44815/Images/ab44815-281493-anti-dna-pkcs-antibody-18-2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs antibody [18-2] (ab44815)ab44815 staining DNA PKcs in wild-type HAP1 cells (top panel) and PRKDC knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab44815 at 1/100 dilution and ab202272 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
