Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)
![Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)](https://www.abcam.com/ps/products/174/ab174573/Images/ab174573-309380-ab133516KOWB.jpg "Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR392] to DNA PKcs - BSA and Azide free
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-DNA PKcs antibody [EPR392] - BSA and Azide free
See all DNA PKcs primary antibodies -
Description
Rabbit monoclonal [EPR392] to DNA PKcs - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: K562, Molt4, MCF7, SH-SY5Y, 293T and PC3 cell lysates. ICC/IF: K-562 cells
-
General notes
Ab174573 is the carrier-free version of ab133516. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab174573 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR392 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)
This WB data was generated using the same anti-DNA PKcs antibody clone, EPR392, in a different buffer formulation (cat# ab133516).
Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: DNA PKcs knockout HAP1 whole cell lysate (40 µg)
Lane 3: K562 whole cell lysate (20 µg)
Lane 4: SHSY5Y whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab133516 observed at 460 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab133516 was shown to specifically react with DNA PKcs when DNA PKcs knockout samples were used. Wild-type and DNA PKcs knockout samples were subjected to SDS-PAGE. Ab133516 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)](https://www.abcam.com/ps/products/174/ab174573/Images/ab174573-3-ab133516ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling DNA PKcs with purified ab133516 at 1:200 dilution (9.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![OI-RD Scanning - Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)](https://www.abcam.com/ps/products/174/ab174573/Images/ab174573-309379-ab133516Affinity.jpg)
OI-RD Scanning - Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)This kD data was generated using the same anti-DNA PKcs antibody clone, EPR392, in a different buffer formulation (cat# ab133516).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)](https://www.abcam.com/ps/products/174/ab174573/Images/ab174573-4-benefits-of-recombinant-antibodies.png)
Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)
