Anti-DNA PKcs antibody (ab70250)
Key features and details
- Rabbit polyclonal to DNA PKcs
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DNA PKcs antibody
See all DNA PKcs primary antibodies -
Description
Rabbit polyclonal to DNA PKcs -
Host species
Rabbit -
Specificity
Ku-80 co-immunoprecipitates using this antibody. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Human DNA PKcs (C terminal). Immunogen is in the region of aa 4075 and the c terminus.
Database link: P78527 -
Positive control
- Whole cell lysate from Hela cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-DNA PKcs antibody (ab70250) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 15 µg
Lane 2 : Whole cell lysate from Hela cells at 50 µg
Predicted band size: 469 kDa
Observed band size: 469 kDa
Additional bands at: 170 kDa, 180 kDa, 230 kDa. We are unsure as to the identity of these extra bands.
-
Immunoprecipitation/ Western Blot of DNA PKcs
Lane 1: ab70250 at 3µg/100µg for IP of HeLa whole cell lysate (1mg) followed by ab70250 at 0.1µg/ml for WB.
Lane 2: Control IgG.
Chemiluminescence with an exposure time of 30 seconds.