Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5670] to DNA PKcs (phospho S2056)
Suitable for: ICC/IF, WB, IHC-P, ELISA
Reacts with: Human

Product name

Anti-DNA PKcs (phospho S2056) antibody [EPR5670]
See all DNA PKcs primary antibodies
Description

Rabbit monoclonal [EPR5670] to DNA PKcs (phospho S2056)
Host species

Rabbit
Specificity

ab124918 only detects DNA PKcs phosphorylated at Serine 2056.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human DNA PKcs aa 2000-2100 (phospho S2056). The exact sequence is proprietary.
Database link: P78527
(Peptide available as ab181446)
Positive control
- WB: Jurkat whole cell lysate (ab7899). IHC-P: Human lung carcinoma and brain tissues. ICC/IF: Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 2.60 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5670
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- Other
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Other

Western blot - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)

All lanes : Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918) at 1/10000 dilution (purified)

Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate - untreated
Lane 2 : Jurkat whole cell lysate - treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 469 kDa
Observed band size: 469 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)

Immunocytochemistry/Immunofluorescence analysis of Jurkat cells (untreated and treated with Alkaline Phosphatase) labelling DNA PKcs (phospho S2056) with purified ab124918 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DNA PKcs (phospho S2056) with purified ab124918 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)

All lanes : Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918) at 1/1000 dilution (unpurified)

Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 469 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling DNA PKcs (phospho S2056) with unpurified ab124918 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)

Serially diluted ab124918 was bound to immobilised phospho- or control peptides (1 microgram per mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.
OI-RD Scanning - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)

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