Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12235(B)] to Clathrin heavy chain
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Clathrin heavy chain antibody [EPR12235(B)]
    See all Clathrin heavy chain primary antibodies

  • Description

    Rabbit monoclonal [EPR12235(B)] to Clathrin heavy chain

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Clathrin heavy chain aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q00610

  • Positive control

    • Hela, SH-SY5Y, A431, NIH/3T3, 293T cell lysates, Human colonic carcinoma tissue, Human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) labeling Clathrin heavy chain with ab172958 at 1/250 (4.1 μg/mL). ab150077, AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) was used as counterstain antibody. Nuclei were stained blue with DAPI.

    Confocal image showing cytoplasmic staining in A431 cells.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) labeling Clathrin heavy chain with ab172958 at 1/250 (4.1 μg/mL). ab150077, AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) was used as counterstain antibody. Nuclei were stained blue with DAPI.

    Confocal image showing cytoplasmic staining in NIH/3T3 cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

    Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    All lanes : Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 191 kDa

  • Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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