Anti-SynGAP antibody [EPR2883Y] (ab77235)
![Anti-SynGAP antibody [EPR2883Y] (ab77235)](https://www.abcam.com/ps/products/77/ab77235/Images/ab77235-264537-anti-syngap-antibody-epr2883y-western-blot.jpg "Anti-SynGAP antibody [EPR2883Y] (ab77235)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2883Y] to SynGAP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SynGAP antibody [EPR2883Y]
See all SynGAP primary antibodies -
Description
Rabbit monoclonal [EPR2883Y] to SynGAP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide within Human SynGAP (C terminal). The exact sequence is proprietary.
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Positive control
- Fetal brain lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2883Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SynGAP antibody [EPR2883Y] (ab77235)All lanes : Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution
Lane 1 : Hippocampus (Rat) Tissue Lysate
Lane 2 : Cortex (Rat) Tissue Lysate
Lane 3 : Hippocampus (Mouse) Tissue Lysate
Lane 4 : Cortex (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 147 kDa
Observed band size: 130,148 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab77235 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
The doublet observed in the Western blot image is consistent with the literature (PMID:9581761).
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![Western blot - Anti-SynGAP antibody [EPR2883Y] (ab77235)](https://www.abcam.com/ps/products/77/ab77235/Images/ab77235-92965-anti-syngap-antibody-epr2883y-western-blot.jpg)
Western blot - Anti-SynGAP antibody [EPR2883Y] (ab77235)Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution + fetal brain lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 147 kDa
Observed band size: 147 kDa
Additional bands at: 50 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
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![Anti-SynGAP antibody [EPR2883Y] (ab77235)](https://www.abcam.com/ps/products/77/ab77235/Images/ab77235-3-benefits-of-recombinant-antibodies.png)
Anti-SynGAP antibody [EPR2883Y] (ab77235)
