Anti-Clathrin heavy chain antibody (ab21679)
Key features and details
- Rabbit polyclonal to Clathrin heavy chain
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Clathrin heavy chain antibody
See all Clathrin heavy chain primary antibodies -
Description
Rabbit polyclonal to Clathrin heavy chain -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB MouseRatHuman
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1650 to the C-terminus of Human Clathrin heavy chain.
Read Abcam's proprietary immunogen policy (Peptide available as ab23440.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Clathrin heavy chain antibody (ab21679)All lanes : Anti-Clathrin heavy chain antibody (ab21679) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : NIH 3T3 whole cell lysate
Lane 4 : PC12 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 180 kDa
Observed band size: 180 kDaBlocking buffer: 2% BSA
Gel type: TA
Exposure Time: 1 minute
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Immunocytochemistry - Anti-Clathrin heavy chain antibody (ab21679)ab21679 staining Clathrin heavy chain in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21679 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-Clathrin heavy chain antibody (ab21679) This image is courtesy of an anonymous AbreviewAnti-Clathrin heavy chain antibody (ab21679) at 1/1000 dilution + HUVEC cell lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Exposure time: 5 seconds
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Immunocytochemistry - Anti-Clathrin heavy chain antibody (ab21679) This image is courtesy of an anonymous AbreviewImmunocytochemical analysis of mouse bEnd.3 labeling Clathrin heavy chain with ab21679 at 1/300 dilution using secondary anti-rabbit-alexa fluor 488 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (ab21679) This image is a courtesy of Anonymous Abreviewab21679 staining Clathrin heavy chain in mouse embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with -20°C ethanol. Samples were incubated with primary antibody at 1/500 dilution (in 0.1% Saponin/1% BSA/PBS) for 1 hour. An Alexa Fluor® 546-conjugated goat polyclonal to rabbit IgG (H+L) was used as secondary antibody at 1/500 dilution. Green color in image show positive staining with Alexa Fluor® 546 conjugated secondary.
