Anti-GAB2 antibody [EP369Y] (ab32365)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP369Y] to GAB2
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-GAB2 antibody [EP369Y]
See all GAB2 primary antibodies -
Description
Rabbit monoclonal [EP369Y] to GAB2 -
Host species
Rabbit -
Specificity
This antibody is predicted to detect the splice isoform 2 based on sequence homology. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human GAB2 aa 600-700. The exact sequence is proprietary.
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Positive control
- WB: K562 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Whole Cell Lysate -
Clonality
Monoclonal -
Clone number
EP369Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) prepared in RIPA lysis method
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
Blocking/Diluting Buffer and concentration:
5% NFDM/TBST -