Mouse Legumain Matched Antibody Pair Kit (ab216790)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 125 pg/ml - 8000 pg/ml
Overview
-
Product name
Mouse Legumain Matched Antibody Pair Kit
See all Legumain kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
24.41 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Legumain Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Legumain.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab207617. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Legumain Capture Antibody 2 x 50µg 1 x 50µg Mouse Legumain Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Legumain Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. -
Tissue specificity
Ubiquitous. Particularly abundant in kidney, heart and placenta. -
Sequence similarities
Belongs to the peptidase C13 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Lysosome. - Information by UniProt
-
Alternative names
- AEP
- Asparaginyl endopeptidase
- cysteine 1
see all -
Database links
- Entrez Gene: 19141 Mouse
- SwissProt: O89017 Mouse
- Unigene: 17185 Mouse
Images
-
Mouse Legumain standard curve.
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Mouse Legumain Matched Antibody Pair Kit (ab216790)To learn more about the advantages of recombinant antibodies see here.
