Anti-Bim antibody (ab7888)
Key features and details
- Rabbit polyclonal to Bim
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Bim antibody
See all Bim primary antibodies -
Description
Rabbit polyclonal to Bim -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Peptide corresponding to amino acids 22 to 40 of human origin. The sequence is identical to that of mouse and differs from that of rat by one amino acid.
-
Positive control
- WB: Human colon and thymus tissue lysates, Rat Myeloma Cell lysate ICC/IF: K562 cells
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Bim antibody (ab7888)All lanes : Anti-Bim antibody (ab7888) at 0.5 µg/ml
Lane 1 : Human thymus tissue lysate
Lane 2 : Human colon tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 23 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bim antibody (ab7888)ab7888 at 20µg/ml staining Bim in human cancer cells by IHC-P
-
Western blot - Anti-Bim antibody (ab7888)Anti-Bim antibody (ab7888) at 0.5 µg/ml + Rat Myeloma Cell lysate at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 23 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody (ab7888)Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed human K562 cells labeling Bim with ab7888 at 20 μg/mL. Goat anti-rabbit IgG secondary antibody at 1/500 dilution was used as the secondry antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody (ab7888)ICC/IF image of ab7888 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7888, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
.JPG?impolicy=product-thumbnail)
