Anti-Bim (phospho S69) antibody (ab131477)
Key features and details
- Rabbit polyclonal to Bim (phospho S69)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bim (phospho S69) antibody
See all Bim primary antibodies -
Description
Rabbit polyclonal to Bim (phospho S69) -
Host species
Rabbit -
Specificity
ab131477 detects endogenous levels of Bim only when phosphorylated at serine 69. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Synthetic phosphopeptide conjugated to KLH, corresponding to a region around the phosphorylation site of serine 69 (
PAS(p)PG
) of Human Bim (UniProt O43521). -
Positive control
- HeLa cells; Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131477 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Bim (phospho S69) antibody (ab131477)Immunofluorescent analysis of methanol-fixed HeLa cells labelling Bim (phospho S69) with ab131477 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bim (phospho S69) antibody (ab131477)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Bim (phospho S69) with ab131477 at 1/50 dilution (left) or ab131477 preincubated with blocking peptide (right).
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