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Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609)

Key features and details

  • Mouse monoclonal [Mab414] to Nuclear Pore Complex Proteins
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Human, Saccharomyces cerevisiae
  • Isotype: IgG1

Overview

  • Product name

    Anti-Nuclear Pore Complex Proteins antibody [Mab414]
    See all Nuclear Pore Complex Proteins primary antibodies

  • Description

    Mouse monoclonal [Mab414] to Nuclear Pore Complex Proteins

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Nuclear pore complex mixture.

  • Epitope

    ab24609 recognizes the conserved domain FXFG repeats in nucleaporins like the p62, p152, p90.

  • Positive control

    • Raw, HEK 293 cell lysate (see Abreview), rat liver lysate (see Aris reference)

  • General notes

     

    This is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.

    This product was changed from ascites to tissue culture supernatant on 20/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.03% Thimerosal (merthiolate)
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Primary antibody notes

    This is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.

  • Clonality

    Monoclonal

  • Clone number

    Mab414

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609) Image courtesy of Saram N J et al. J Biol Chem. 2011 Nov 11; 286(45): 38989–39001. doi: 10.1074/jbc.M111.297952

    ab24609 staining nuclear pore complex proteins in Human chronic myelogenous leukemia cells from bone marrow cells. Cells were fixed with 4% paraformaldehyde in DPBS buffer for 20 mins and permeabilised with 0.1% Triton X-100 for 20 min at room temperature. Cells were blocked with 2% normal goat serum in DPBS with 1% BSA and washed with 0.1% Tween 20.

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609) This image is courtesy of an Abreview submitted by Dr Kirk McManus

    ab24609 (1/200) staininig nuclear pore complex proteins in human RPE-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100 and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.

    This image was generated using the ascites version of the product.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609) Image and protocol kindly provided by Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome

    ab24609 (1/500) staining nuclear pore complex proteins in human Hela Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T°C) or Methanol (6 min in Methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609) Image and protocol kindly provided by Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome

    ab24609 (1/500) staining nuclear pore complex proteins in murine NIH/3T3 Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T°C) or Methanol (6 min in Methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).

    This image was generated using the ascites version of the product.

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