Anti-Nuclear Pore Complex Proteins antibody [39C7] (ab60080)
![Anti-Nuclear Pore Complex Proteins antibody [39C7] (ab60080)](https://www.abcam.com/ps/products/60/ab60080/Images/ab60080-73887-anti-nuclear-pore-complex-proteins-antibody-39c7-flow-cytometry.jpg "Anti-Nuclear Pore Complex Proteins antibody [39C7] (ab60080)")
Key features and details
- Mouse monoclonal [39C7] to Nuclear Pore Complex Proteins
- Suitable for: Flow Cyt
- Reacts with: Human, Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-Nuclear Pore Complex Proteins antibody [39C7]
See all Nuclear Pore Complex Proteins primary antibodies -
Description
Mouse monoclonal [39C7] to Nuclear Pore Complex Proteins -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Saccharomyces cerevisiae -
Immunogen
Yeast nuclear preparation
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
39C7 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Nuclear Pore Complex Proteins antibody [39C7] (ab60080)Overlay histogram showing HeLa cells stained with ab60080 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60080, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
