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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation

Anti-PRMT5 antibody - N-terminal (ab227457)

Anti-PRMT5 antibody - N-terminal (ab227457)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PRMT5 - N-terminal
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PRMT5 antibody - N-terminal
    See all PRMT5 primary antibodies
  • Description

    Rabbit polyclonal to PRMT5 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PRMT5 (N terminal). The exact sequence is proprietary.
    Database link: O14744

  • Positive control

    • IHC-P: Mouse intestine and kidney tissues; MCF7 xenograft tissue. ICC/IF: A431 cells. WB: Raji whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Arginine Methylation

Images

  • Western blot - Anti-PRMT5 antibody - N-terminal (ab227457)
    Western blot - Anti-PRMT5 antibody - N-terminal (ab227457)
    Anti-PRMT5 antibody - N-terminal (ab227457) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 73 kDa



    7.5% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT5 antibody - N-terminal (ab227457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT5 antibody - N-terminal (ab227457)

    Paraffin-embedded mouse intestine tissue stained for PRMT5 using ab227457 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-PRMT5 antibody - N-terminal (ab227457)
    Immunocytochemistry/ Immunofluorescence - Anti-PRMT5 antibody - N-terminal (ab227457)

    A431 (human epithelial cell line from cervix adenocarcinoma) cells stained for PRMT5 (green) using ab227457 at 1/500 dilution in ICC/IF. Cells were fixed in paraformaldehyde.

    Lower panel costained with Hoechst 33342.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT5 antibody - N-terminal (ab227457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT5 antibody - N-terminal (ab227457)

    Paraffin-embedded MCF7 (human) xenograft tissue stained for PRMT5 using ab227457 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT5 antibody - N-terminal (ab227457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT5 antibody - N-terminal (ab227457)

    Paraffin-embedded mouse kidney tissue stained for PRMT5 using ab227457 at 1/500 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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