Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRMT5 antibody - C-terminal (ab31751)

Key features and details

  • Rabbit polyclonal to PRMT5 - C-terminal
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Xenopus laevis
Zebrafish
Application Abreviews Notes
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

ab31751 shows weak staining in mouse samples.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

ab31751 shows weak staining in mouse samples.

Target

  • Function

    Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.

  • Post-translational
    modifications

    Disulfide bonds and non-covalent association mediate homooligomers formation.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • 72 kDa ICln binding protein antibody
      • 72 kDa ICln-binding protein antibody
      • ANM5_HUMAN antibody
      • Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
      • Histone-arginine N-methyltransferase PRMT5 antibody
      • HMT1 hnRNP methyltransferase like 5 antibody
      • HOMOLOG OF; SKB1 antibody
      • HRMT1L5 antibody
      • IBP72 antibody
      • Jak-binding protein 1 antibody
      • JBP 1 antibody
      • JBP1 antibody
      • PRMT 5 antibody
      • PRMT5 antibody
      • Protein arginine methyltransferase 5 antibody
      • Protein arginine N methyltransferase 5 antibody
      • Protein arginine N methyltransferase 5 N terminally processed antibody
      • Protein arginine N-methyltransferase 5 antibody
      • S. POMBE antibody
      • S. POMBE HOMOLOG OF; SKB1 antibody
      • SHK1 KINASE BINDING PROTEIN 1 antibody
      • Shk1 kinase binding protein 1 homolog antibody
      • Shk1 kinase-binding protein 1 homolog antibody
      • Shk1 kinase/binding protein 1, S. pombe, homolog of antibody
      • SKB 1 antibody
      • SKB1 antibody
      • SKB1 homolog antibody
      • SKB1: SKB1 homolog (S. pombe) antibody
      • SKB1Hs antibody
      see all

    Images

    • Western blot - Anti-PRMT5 antibody - C-terminal (ab31751)
      Western blot - Anti-PRMT5 antibody - C-terminal (ab31751)
      All lanes : Anti-PRMT5 antibody - C-terminal (ab31751) at 1 µg/ml

      Lane 1 : HL60 (Human promyelocytic leukemia cell line) whole cell lysate
      Lane 2 : HEK-293 (Human embryonic kidney cell line) whole cell lysate
      Lane 3 : NIH/3T3 whole cell lysate (ab7179)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 72 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 110 kDa, 37 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 6 minutes

    References (12)

    Publishing research using ab31751? Please let us know so that we can cite the reference in this datasheet.

    ab31751 has been referenced in 12 publications.

    • Webb LM  et al. NF-?B/mTOR/MYC Axis Drives PRMT5 Protein Induction After T Cell Activation via Transcriptional and Non-transcriptional Mechanisms. Front Immunol 10:524 (2019). PubMed: 30941147
    • Bernkopf DB  et al. Sulforaphane inhibits growth and blocks Wnt/ß-catenin signaling of colorectal cancer cells. Oncotarget 9:33982-33994 (2018). PubMed: 30338040
    • Hartmann H  et al. Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life Sci Alliance 1:e201800070 (2018). PubMed: 30456350
    • Muhammad AB  et al. Menin and PRMT5 suppress GLP1 receptor transcript and PKA-mediated phosphorylation of FOXO1 and CREB. Am J Physiol Endocrinol Metab 313:E148-E166 (2017). PubMed: 28270438
    • Webb LM  et al. PRMT5-Selective Inhibitors Suppress Inflammatory T Cell Responses and Experimental Autoimmune Encephalomyelitis. J Immunol 198:1439-1451 (2017). WB ; Mouse . PubMed: 28087667
    View all Publications for this product

    Images

    • Western blot - Anti-PRMT5 antibody - C-terminal (ab31751)
      Western blot - Anti-PRMT5 antibody - C-terminal (ab31751)
      All lanes : Anti-PRMT5 antibody - C-terminal (ab31751) at 1 µg/ml

      Lane 1 : HL60 (Human promyelocytic leukemia cell line) whole cell lysate
      Lane 2 : HEK-293 (Human embryonic kidney cell line) whole cell lysate
      Lane 3 : NIH/3T3 whole cell lysate (ab7179)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 72 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 110 kDa, 37 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 6 minutes

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