Anti-PRMT5 antibody (ab183068)
Key features and details
- Rabbit polyclonal to PRMT5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRMT5 antibody
See all PRMT5 primary antibodies -
Description
Rabbit polyclonal to PRMT5 -
Host species
Rabbit -
Specificity
ab183068 detects endogenous level of total PRMT5 protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human PRMT5 aa 600 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14744 -
Positive control
- K562, HeLa and 293T cell lysates; Human breast cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
(PBS is without Mg2+ and Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PRMT5 antibody (ab183068)All lanes : Anti-PRMT5 antibody (ab183068) at 1/400 dilution
Lane 1 : K562 cell lsyate
Lane 2 : HeLa cell lsyate
Lane 3 : 293T cell lsyate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP)
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 2 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT5 antibody (ab183068)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling PRMT5 with ab183068 at a 1/20 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)
