Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7738] to Methylmalonyl Coenzyme A mutase
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738]
    See all Methylmalonyl Coenzyme A mutase primary antibodies

  • Description

    Rabbit monoclonal [EPR7738] to Methylmalonyl Coenzyme A mutase

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

     

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Methylmalonyl Coenzyme A mutase aa 50-150 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, K-562, 293T and human fetal liver lysates; mouse and rat brain tissue lysates, human colon and kidney tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Methylmalonyl Coenzyme A mutase knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: Human liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab134956 (unpurified) observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab134956 was shown to specifically react with Methylmalonyl Coenzyme A mutase when Methylmalonyl Coenzyme A mutase knockout samples were used. Wild-type and Methylmalonyl Coenzyme A mutase knockout samples were subjected to SDS-PAGE. ab134956 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Methylmalonyl Coenzyme A mutase with purified ab134956 at 1:50 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956) at 1/2000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Methylmalonyl Coenzyme A mutase with ab134956 (unpurified) at 1/50 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Human fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 83 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Methylmalonyl Coenzyme A mutase with ab134956 (unpurified) at dilution 1/50.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

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