Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)
Key features and details
- Mouse polyclonal to Methylmalonyl Coenzyme A mutase
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Methylmalonyl Coenzyme A mutase antibody
See all Methylmalonyl Coenzyme A mutase primary antibodies -
Description
Mouse polyclonal to Methylmalonyl Coenzyme A mutase -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length Methylmalonyl Coenzyme A mutase protein (Human)
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Positive control
- WB: Human liver tissue lysate, Methylmalonyl Coenzyme A mutase transfected 293T cell lysate, Euglena whole cell lysate. ICC: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution + human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
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Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution
Lane 1 : Methylmalonyl Coenzyme A mutase transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)ICC/IF image of ab67869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67869, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/2000 dilution
Lane 1 : Euglena whole cell lysate with Milk and 0.05% Tween20
Lane 2 : Euglena (RNAi gene silencing Methylmalonyl Coenzyme A mutase) whole cell lysate, with Milk and 0.05% Tween20
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG(H+L) HRP conjugated at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 83 kDa
Exposure time: 10 minutes
