Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y175] to Hsp27 (phospho S78) - BSA and Azide free
  • Suitable for: IHC-P, WB, Dot blot, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free
    See all Hsp27 primary antibodies

  • Description

    Rabbit monoclonal [Y175] to Hsp27 (phospho S78) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises Hsp27 (phospho S78). It will detect Src phosphorylation on Serine 78.

  • Tested applications

    Suitable for: IHC-P, WB, Dot blot, ICCmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247262 is the carrier-free version of ab32501 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247262 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    All lanes : Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates, treated with anisomycin.
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates, treated with anisomycin. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32501, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    Immunocytochemistry - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    This data was developed using ab32501, the same antibody clone in a different buffer formulation.ab32501 staining Hsp27 in HeLa cells by Immunocytochemistry. Tissue was fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (4.5 μg/ml). ab150077 AlexaFluor®488 Goat anti-Rabbit (1/1000) was used as the secondary antibody. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 2.5 μg/ml and DAPI were used as counter stains. Confocal image showing the expression was increased after treatment with anisomycin (25ug/ml for 30min) and then decreased after treatment with the Lambda Protein Phosphatase 31? for 2h
  • Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    This data was developed using ab32501, the same antibody clone in a different buffer formulation.Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32501 at dilution 1/2000.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    This data was developed using ab32501, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Hsp27 phospho S78 expression in paraffin embedded human breast carcinoma using 1/250 ab32501. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    Immunocytochemistry - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    This data was developed using ab32501, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Hsp27 phospho S78 expression in HeLa cells using 1/250 ab32501.
  • Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
    Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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