Anti-Hsp90 antibody [Hyb-K41220A] (ab241995)
![Anti-Hsp90 antibody [Hyb-K41220A] (ab241995)](https://www.abcam.com/ps/products/241/ab241995/Images/ab241995-331804-95bc.jpg "Anti-Hsp90 antibody [Hyb-K41220A] (ab241995)")
Key features and details
- Mouse monoclonal [Hyb-K41220A] to Hsp90
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Hsp90 antibody [Hyb-K41220A]
See all Hsp90 primary antibodies -
Description
Mouse monoclonal [Hyb-K41220A] to Hsp90 -
Host species
Mouse -
Specificity
Will detect both alpha (inducible) and beta (constitutively-expressed) forms.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB MouseRatHuman
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Immunogen
Recombinant fragment corresponding to Human Hsp90. Specificity mapped to amino acids 291-304.
Database link: P07900 -
Epitope
Specificity mapped to amino acids 291-304. -
Positive control
- WB: A431, A594, HCT 116, HeLa, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3 and T98G cell lysates; rat brain tissue lysate. ICC/IF: HeLa cells. IHC-P: Mouse backskin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Hyb-K41220A -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [Hyb-K41220A] (ab241995)
Bouin's fixed, paraffin-embedded mouse back skin tissue stained for Hsp90 using ab241995 at 1/100 dilution for 1 hr at RT in immunohistochemical analysis.
Secondary antibody is FITC Goat Anti-Mouse at 1/50 dilution for 1 hr at RT.
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![Western blot - Anti-Hsp90 antibody [Hyb-K41220A] (ab241995)](https://www.abcam.com/ps/products/241/ab241995/Images/ab241995-331805-95c.png)
Western blot - Anti-Hsp90 antibody [Hyb-K41220A] (ab241995)All lanes : Anti-Hsp90 antibody [Hyb-K41220A] (ab241995) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 7 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 11 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Lane 12 : T98G (Human glioblastoma multiforme) cell lysate
Lane 13 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
Lanes 1-5 & 7-13 : Sheep Anti-Mouse IgG: HRP
Predicted band size: 85 kDaPrimary incubation for 2 hours at RT.
Secondary incubation for 1 hour at RT.
Block: 1.5% BSA for 30 minutes at RT.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [Hyb-K41220A] (ab241995)](https://www.abcam.com/ps/products/241/ab241995/Images/ab241995-331803-95a.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [Hyb-K41220A] (ab241995)2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp90 (green, B) using ab241995 at 1/100 dilution for 12 hours at 4°C in ICC/IF. Secondary antibody is Goat anti-Mouse FITC at 1/200 dilution. The nuclear counterstain is DAPI (blue, A). (C) is a composite of (A) and (B).
