Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HSP27S82-CB2] to Hsp27 (phospho S82)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2]
    See all Hsp27 primary antibodies

  • Description

    Rabbit monoclonal [HSP27S82-CB2] to Hsp27 (phospho S82)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Hsp27 (phospho S82). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P04792

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa (treated with UV+ TPA) cell extract. Flow cyt: HeLa cells (treated with UV + TPA).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)
    Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)
    Flow cytometric analysis of HeLa cells unstained and untreated cells as as negative control (blue) or untreated (red) or treated with UV and TPA (green) using ab278625 at 1.0 ng/mL.
  • Western blot - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)
    Western blot - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)
    All lanes : Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625) at 0.005 µg/ml

    Lane 1 : HeLa cell extract treated with UV plus TPA
    Lane 2 : HeLa cell extract untreated

    Predicted band size: 23 kDa

  • Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)
    Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)
    Peptide blocking flow cytometric analysis of HeLa cells unstained and untreated as negative control (light blue) or stained and untreated (red) or treated with UV+ plus TPA (green) or untreated and blocked with phospho-peptide (black) or UV plus TPA blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV plus TPA and blocked with non-phospho peptide (purple) using Phospho-HSP27 (Ser82) antibody ab278625 at 10 µg/mL.
  • Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)
    Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.