Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)
![Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)](https://www.abcam.com/ps/products/278/ab278625/Images/ab278625-3-hsp27s82cb21ngml.jpg "Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HSP27S82-CB2] to Hsp27 (phospho S82)
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2]
See all Hsp27 primary antibodies -
Description
Rabbit monoclonal [HSP27S82-CB2] to Hsp27 (phospho S82) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human Hsp27 (phospho S82). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P04792 -
Positive control
- WB: HeLa (treated with UV+ TPA) cell extract. Flow cyt: HeLa cells (treated with UV + TPA).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
HSP27S82-CB2 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)Flow cytometric analysis of HeLa cells unstained and untreated cells as as negative control (blue) or untreated (red) or treated with UV and TPA (green) using ab278625 at 1.0 ng/mL.
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![Western blot - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)](https://www.abcam.com/ps/products/278/ab278625/Images/ab278625-453844-anti-hsp27-western-blot-hela-human.jpg)
Western blot - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)All lanes : Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625) at 0.005 µg/ml
Lane 1 : HeLa cell extract treated with UV plus TPA
Lane 2 : HeLa cell extract untreated
Predicted band size: 23 kDa
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![Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)](https://www.abcam.com/ps/products/278/ab278625/Images/ab278625-4-hsp28s82cb2peptideblocking10ngml.jpg)
Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)Peptide blocking flow cytometric analysis of HeLa cells unstained and untreated as negative control (light blue) or stained and untreated (red) or treated with UV+ plus TPA (green) or untreated and blocked with phospho-peptide (black) or UV plus TPA blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV plus TPA and blocked with non-phospho peptide (purple) using Phospho-HSP27 (Ser82) antibody ab278625 at 10 µg/mL. -
![Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)](https://www.abcam.com/ps/products/278/ab278625/Images/ab278625-1-benefits-of-recombinant-antibodies.png)
Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278625)