Anti-Hsp47 antibody [1C4-1A6] (ab242006)
![Anti-Hsp47 antibody [1C4-1A6] (ab242006)](https://www.abcam.com/ps/products/242/ab242006/Images/ab242006-336321-anti-hsp47-antibody-1c4-1a6-western-blot.jpg "Anti-Hsp47 antibody [1C4-1A6] (ab242006)")
Key features and details
- Mouse monoclonal [1C4-1A6] to Hsp47
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp47 antibody [1C4-1A6]
See all Hsp47 primary antibodies -
Description
Mouse monoclonal [1C4-1A6] to Hsp47 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Hsp47. NP_001193943
Database link: P50454 -
Positive control
- WB:
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
1C4-1A6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Hsp47 antibody [1C4-1A6] (ab242006)Lane 1 : Anti-Hsp47 antibody [1C4-1A6] (ab242006) at 1/250 dilution
Lane 2 : Anti-Hsp47 antibody [1C4-1A6] (ab242006) at 1/500 dilution
Lane 3 : Anti-Hsp47 antibody [1C4-1A6] (ab242006) at 1/1000 dilution
All lanes : A431 (human epidermoid carcinoma cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP Goat Anti-Mouse at 1/50 dilution
Predicted band size: 46 kDaPrimary incubation for 1 hour at room temperature.
Block: 5% milk + TBST for 1 hour at room temperature.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (ab242006)](https://www.abcam.com/ps/products/242/ab242006/Images/ab242006-336320-anti-hsp47-antibody-1c4-1a6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (ab242006)2% paraformaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp47 (red, B) using ab242006 at 1/100 dilution in ICC/IF. The nuclear counter stain is DAPI (blue, A). Panel C shows the merged images.
Primary incubation for 12 hours at 4°.
Secondary antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT.
Cell were heat shocked at 42°C for 1 hour.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (ab242006)](https://www.abcam.com/ps/products/242/ab242006/Images/ab242006-336319-anti-hsp47-antibody-1c4-1a6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (ab242006)2% paraformaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp47 (green, B) using ab242006 at 1/100 dilution in ICC/IF. The nuclear counter stain is DAPI (blue, A). Panel C shows the merged images.
Primary incubation for 12 hours at 4°.
Secondary antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT.
Cell were heat shocked at 42°C for 1 hour.
