Anti-Hsp47 antibody [134CT7.1.8] (ab86750)
![Anti-Hsp47 antibody [134CT7.1.8] (ab86750)](https://www.abcam.com/ps/products/86/ab86750/Images/ab86750-300884-anti-hsp47-antibody-134ct718-western-blot.jpg "Anti-Hsp47 antibody [134CT7.1.8] (ab86750)")
Key features and details
- Mouse monoclonal [134CT7.1.8] to Hsp47
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp47 antibody [134CT7.1.8]
See all Hsp47 primary antibodies -
Description
Mouse monoclonal [134CT7.1.8] to Hsp47 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following lysates: NIH3T3; Human Placenta
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
134CT7.1.8 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Hsp47 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab86750 observed at 46 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab86750 was shown to recognize Hsp47 in wild-type HAP1 cells as signal was lost at the expected MW in Hsp47 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Hsp47 knockout samples were subjected to SDS-PAGE. ab86750 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 5 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)](https://www.abcam.com/ps/products/86/ab86750/Images/ab86750-228029-anti-hsp47-antibody-134ct718-western-blot.jpg)
Western blot - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)All lanes : Anti-Hsp47 antibody [134CT7.1.8] (ab86750) at 5 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 4 minutes
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![Flow Cytometry - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)](https://www.abcam.com/ps/products/86/ab86750/Images/ab86750-101820-anti-hsp47-antibody-134ct718-flow-cytometry.jpg)
Flow Cytometry - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)Overlay histogram showing HL60 cells stained with ab86750 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86750, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
