Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593)
Key features and details
- Rabbit polyclonal to elF2 alpha (phospho S52) - N-terminal
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-elF2 alpha (phospho S52) antibody - N-terminal -
Description
Rabbit polyclonal to elF2 alpha (phospho S52) - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr HumanWB RatHuman
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Immunogen
Synthetic peptide within Human elF2 alpha (N terminal) (phospho S52). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P05198 -
Positive control
- WB: HEK-293T whole cell extract treated with/without anisomycin; PC-12 and HepG2 whole cell extracts treated with/without thapsigargin; HeLa whole cell extract treated with/without tunicamycin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593)All lanes : Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 4 µg/ml tunicamycin for 16 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa10% SDS-PAGE gel.
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Western blot - Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593)All lanes : Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 3 µM thapsigargin for 12 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa10% SDS-PAGE gel.
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Western blot - Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593)All lanes : Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) treated with 300 µM thapsigargin for 8 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa10% SDS-PAGE gel.
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Western blot - Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593)All lanes : Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) treated with 25 µg/ml anisomycin for 16 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa10% SDS-PAGE gel.