Anti-Grp75/MOT antibody (ab53098)
Key features and details
- Rabbit polyclonal to Grp75/MOT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Grp75/MOT antibody
See all Grp75/MOT primary antibodies -
Description
Rabbit polyclonal to Grp75/MOT -
Host species
Rabbit -
Specificity
Detects endogenous levels of total Grp75 / MOT protein -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human Grp75/MOT aa 630-679.
Database link: P38646 -
General notes
Previously labelled as Grp75.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Grp75/MOT antibody (ab53098)All lanes : Anti-Grp75/MOT antibody (ab53098) at 1/300 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with peptide
Predicted band size: 74 kDa
Observed band size: 74 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (ab53098)Immunohistochemical analysis of Grp75/MOT in paraffin-embedded human breast carcinoma tissue, using ab53098 at 1/50 dilution, in the presence (right) or absence (left) of peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (ab53098)Immunocytochemistry/ Immunofluorescence analysis of Grp75/MOT in COS-7 (African green monkey kidney fibroblast-like) cells, using ab53098 at 1/100 dilution, in the presence (right) or absence (left) of peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (ab53098)ICC/IF image of ab53098 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53098, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
