Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Grp75/MOT antibody [9F8] (ab171089)

Key features and details

Mouse monoclonal [9F8] to Grp75/MOT
Suitable for: WB, ICC/IF, IP
Reacts with: Mouse, Rat, Human, Non human primates, African green monkey
Isotype: IgG

Product name

Anti-Grp75/MOT antibody [9F8]
See all Grp75/MOT primary antibodies
Description

Mouse monoclonal [9F8] to Grp75/MOT
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Mouse Rat Human African green monkey

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Grp75/MOT. Expressed in 293T cells.
Database link: Q8N1C8
Positive control
- 293T, HeLa, K562, A431, COS7 and NRK lysates; HeLa cells.
General notes

Previously labelled as Grp75.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol (glycerin, glycerine), 69% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

9F8
Isotype

IgG
Research areas

Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)

All lanes : Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

Lane 1 : Untransfected HeLa cells
Lane 2 : HeLa cells transfected with scrambled siRNA
Lane 3 : HeLa cells transfected with Grp75/MOT siRNA

Secondary
All lanes : Goat anti-Mouse IgG (H+L) HRP conjugate at 1/4000 dilution

Predicted band size: 74 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089)

Immunofluorescence analysis of Grp75/MOT was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab171089 at 1:50 dilution in 0.1% BSA, incubated at 4ºC overnight and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)

All lanes : Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Hek293 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : MCF-7 cell lysate
Lane 7 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 8 : L6 cell lysate
Lane 9 : Rat brain cell lysate
Lane 10 : Rat skeletal muscle cell lysate
Lane 11 : Rat liver cell lysate

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : Goat anti-Mouse IgG (H+L) HRP conjugate at 1/4000 dilution

Predicted band size: 74 kDa
Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)

All lanes : Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : NIH/3T3 whole cell lysate
Lane 7 : NRK whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : Goat anti-Mouse HRP at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 74 kDa

4-20% Tris-HCL polyacrylamide gel
Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089)

Immunofluorescent analysis of HeLa cells labeling Grp75/MOT with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells.
Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (ab171089)

Detection of Grp75/MOT in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2µg per 750µg lysate. Detected with ab171089 at 1/1000 dilution.

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