Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Grp75/MOT antibody [9F8] (ab171089)

Key features and details

  • Mouse monoclonal [9F8] to Grp75/MOT
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human, Non human primates, African green monkey
  • Isotype: IgG

Overview

  • Product name

    Anti-Grp75/MOT antibody [9F8]
    See all Grp75/MOT primary antibodies

  • Description

    Mouse monoclonal [9F8] to Grp75/MOT

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    African green monkey
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Grp75/MOT. Expressed in 293T cells.
    Database link: Q8N1C8

  • Positive control

    • 293T, HeLa, K562, A431, COS7 and NRK lysates; HeLa cells.

  • General notes

    Previously labelled as Grp75. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)
    Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)
    All lanes : Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

    Lane 1 : Untransfected HeLa cells
    Lane 2 : HeLa cells transfected with scrambled siRNA
    Lane 3 : HeLa cells transfected with Grp75/MOT siRNA

    Secondary
    All lanes : Goat anti-Mouse IgG (H+L) HRP conjugate at 1/4000 dilution

    Predicted band size: 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089)
    Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089)

    Immunofluorescence analysis of Grp75/MOT was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab171089 at 1:50 dilution in 0.1% BSA, incubated at 4ºC overnight and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

  • Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)
    Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)
    All lanes : Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Hek293 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : HepG2 cell lysate
    Lane 6 : MCF-7 cell lysate
    Lane 7 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
    Lane 8 : L6 cell lysate
    Lane 9 : Rat brain cell lysate
    Lane 10 : Rat skeletal muscle cell lysate
    Lane 11 : Rat liver cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG (H+L) HRP conjugate at 1/4000 dilution

    Predicted band size: 74 kDa

  • Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)
    Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089)
    All lanes : Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : A431 whole cell lysate
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 6 : NIH/3T3 whole cell lysate
    Lane 7 : NRK whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 74 kDa



    4-20% Tris-HCL polyacrylamide gel

  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089)
    Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (ab171089)

    Immunofluorescent analysis of HeLa cells labeling Grp75/MOT with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells. 

  • Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (ab171089)
    Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (ab171089)

    Detection of Grp75/MOT in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2µg per 750µg lysate. Detected with ab171089 at 1/1000 dilution.

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