Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Lin28A antibody [EPR4640] (ab124765)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4640] to Lin28A
Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-Lin28A antibody [EPR4640]
See all Lin28A primary antibodies
Description

Rabbit monoclonal [EPR4640] to Lin28A
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

corresponding to Human Lin28A.
Positive control
- WB: NCCIT and JAR cell lysates IP: NCCIT cell lysates Flow Cyt: NCCIT cells ICC/IF: JAR cells IHC-P: human liver tissue, human seminoma tissue.
General notes
Mouse and Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4640
Isotype

IgG
Research areas

Western blot - Anti-Lin28A antibody [EPR4640] (ab124765)

All lanes : Anti-Lin28A antibody [EPR4640] (ab124765) at 1/10000 dilution (Purified)

Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates
Lane 2 : JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 26 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] (ab124765)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Lin28A with purified ab124765 at 1/50 dilution (12 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Lin28A antibody [EPR4640] (ab124765)

All lanes : Anti-Lin28A antibody [EPR4640] (ab124765) at 1/10000 dilution (unpurified)

Lane 1 : NCCIT cell lysate
Lane 2 : JAR cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (ab124765)

Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/500 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-Lin28A antibody [EPR4640] (ab124765)

Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-Lin28A antibody [EPR4640] (ab124765)

ab124765 (purified) at 1/30 dilution (2ug) immunoprecipitating Lin28A in NCCIT whole cell lysate. NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab124765 & NCCIT whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124765 in NCCIT whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] (ab124765)

ab124765 (unpurified), at 1/100 dilution, staining Lin28A in paraffin-embedded Human seminoma tissue by Immunohistochemistry.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (ab124765)

ab124765 (unpurified), at 1/100 dilution, staining Lin28A in NCCIT cells by Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] (ab124765)

ab124765 (unpurified) showing positive staining in Glioma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] (ab124765)

ab124765 (unpurified) showing positive staining in Dysgerminoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] (ab124765)

ab124765 (unpurified) showing positive staining in Embryonal carcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] (ab124765)

ab124765 (unpurified) showing negative staining in Normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-Lin28A antibody [EPR4640] (ab124765)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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