Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Arfaptin-1 antibody [EPR16360] (ab191430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16360] to Arfaptin-1
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Arfaptin-1 antibody [EPR16360]
    See all Arfaptin-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16360] to Arfaptin-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2, BxPC-3, Jurkat, Human fetal liver, Mouse brain, Rat brain and Raw264.7 lysates; Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)
    Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)
    All lanes : Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : HepG2 lysate
    Lane 3 : BxPC-3 lysate
    Lane 4 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)
    Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)
    Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/10000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa
    Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)
    Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)
    All lanes : Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Raw264.7 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.

  • Flow Cytometry - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)
    Flow Cytometry - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)

    Flow Cytometrical analysis of Jurkat cells labeling Arfaptin-1 with ab191430 at 1/120 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.

  • Anti-Arfaptin-1 antibody [EPR16360] (ab191430)
    Anti-Arfaptin-1 antibody [EPR16360] (ab191430)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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