Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Arfaptin-1 antibody [EPR16360] (ab191430)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16360] to Arfaptin-1
Suitable for: Flow Cyt, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Arfaptin-1 antibody [EPR16360]
See all Arfaptin-1 primary antibodies
Description

Rabbit monoclonal [EPR16360] to Arfaptin-1
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Mouse

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, HepG2, BxPC-3, Jurkat, Human fetal liver, Mouse brain, Rat brain and Raw264.7 lysates; Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16360
Isotype

IgG
Research areas

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)

All lanes : Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/10000 dilution

Lane 1 : HeLa lysate
Lane 2 : HepG2 lysate
Lane 3 : BxPC-3 lysate
Lane 4 : Jurkat lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)

Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/10000 dilution + Human fetal liver lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)

All lanes : Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Raw264.7 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometry - Anti-Arfaptin-1 antibody [EPR16360] (ab191430)

Flow Cytometrical analysis of Jurkat cells labeling Arfaptin-1 with ab191430 at 1/120 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.
Anti-Arfaptin-1 antibody [EPR16360] (ab191430)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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