Anti-IDH1 (mutated R132S) antibody [SMab-1] (ab264049)
![Anti-IDH1 (mutated R132S) antibody [SMab-1] (ab264049)](https://www.abcam.com/ps/products/264/ab264049/Images/ab264049-393925-anti-idh1-mutated-r132-s-antibody-smab1-western-blot-histagged-human-idh1.jpg "Anti-IDH1 (mutated R132S) antibody [SMab-1] (ab264049)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [SMab-1] to IDH1 (mutated R132S)
- Suitable for: WB, ELISA
- Reacts with: Human
Overview
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Product name
Anti-IDH1 (mutated R132S) antibody [SMab-1]
See all IDH1 primary antibodies -
Description
Mouse monoclonal [SMab-1] to IDH1 (mutated R132S) -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SMab-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-IDH1 (mutated R132S) antibody [SMab-1] (ab264049)
ab264049 used at 0.949µg/ml.
Goat Anti-Rat IgG (H+L), HRP) (ab205720) used as the secondary at a 1/10,000 dilution.
Blocking/Dilution buffer:5% NFDM/TBST.
Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng
The loading samples are E.coil extracts containing recombinant protein respectively.
Exposure time of 3 mins. -
![ELISA - Anti-IDH1 (mutated R132S) antibody [SMab-1] (ab264049)](https://www.abcam.com/ps/products/264/ab264049/Images/ab264049-393926-AntiIDH1-mutated-R132-S-antibody-SMab1-elisa2.jpg)
ELISA - Anti-IDH1 (mutated R132S) antibody [SMab-1] (ab264049)ELISA - Anti-IDH1 (mutated R132 S) antibody [SMab-1].
ab264049 used at 0-5000 ng/ml.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.
Antigen concentration 1000 ng/ml.
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![Anti-IDH1 (mutated R132S) antibody [SMab-1] (ab264049)](https://www.abcam.com/ps/products/264/ab264049/Images/ab264049-3-benefits-of-recombinant-antibodies.png)
Anti-IDH1 (mutated R132S) antibody [SMab-1] (ab264049)
