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Signal Transduction Metabolism Energy Metabolism

Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)

Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12376] to GANAB - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-GANAB antibody [EPR12376] - BSA and Azide free
    See all GANAB primary antibodies
  • Description

    Rabbit monoclonal [EPR12376] to GANAB - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249921 is the carrier-free version of ab176349 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249921 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as alpha Glucosidase II

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12376
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Images

  • Western blot - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    Western blot - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)

    This data was developed using ab176349, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: GANAB knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab176349 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab176349 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. ab176349 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    Flow Cytometry - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    This data was developed using ab176349, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Hela cells labeling GANAB with ab176349 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
  • Immunocytochemistry - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    Immunocytochemistry - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    This data was developed using ab176349, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells labeling GANAB with ab176349 at 1/50 dilution (red). DAPI nuclear staining (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    This data was developed using ab176349, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GANAB with ab176349 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    This data was developed using ab176349, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Humangastric carcinoma tissue labeling GANAB with ab176349 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    Western blot - Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    All lanes : Anti-GANAB antibody [EPR12376] (ab176349) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human placenta lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 107 kDa



    This data was developed using ab176349, the same antibody clone in a different buffer formulation.

     

  • Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)
    Anti-GANAB antibody [EPR12376] - BSA and Azide free (ab249921)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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