Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22170] to SLAMF6 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free
    See all SLAMF6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22170] to SLAMF6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and Ramos whole cell lysates; Human lymph node and tonsil lysates. ICC/IF: Jurkat and Ramos cells. Flow Cyt: Ramos and human peripheral blood mononuclear cells. IP: Jurkat and Ramos whole cell lysates.

  • General notes

    Ab238421 is the carrier-free version of ab224201. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238421 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22170

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)
    Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling SLAMF6 with ab224201 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

  • Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)
    Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)

    SLAMF6 was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab224201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Ramos whole cell lysate 10 µg (Input).
    Lane 2: ab224201 IP in Ramos whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224201 in Ramos whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This blot was developed using a high sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

  • Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)
    Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)

    SLAMF6 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab224201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224201 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Jurkat whole cell lysate 10 µg (Input).
    Lane 2: ab224201 IP in Jurkat whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224201 in Jurkat whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This blot was developed using a high sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

  • Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)
    Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)

    Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling SLAMF6 with ab224201 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

  • Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)
    Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling SLAMF6 with ab224201 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in the Jurkat cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

  • Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)
    Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (human Burkitt's lymphoma cell line) cells labeling SLAMF6 with ab224201 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in the Ramos cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

  • Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)
    Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (human Burkitt's lymphoma cell line) cells labeling SLAMF6 with ab224201 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in the Ramos cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab224201).

  • Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)
    Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (ab238421)

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