Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23122-130] to SLAMF6
  • Suitable for: ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-SLAMF6 antibody [EPR23122-130]
    See all SLAMF6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23122-130] to SLAMF6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: EL4 and mouse splenocytes cells. Flow Cyt: Mouse splenocytes cells. IP: Mouse spleen lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23122-130

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)
    Immunoprecipitation - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

    SLAMF6 was immunoprecipitated from 0.35 mg Mouse spleen lysate with ab243658 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243658 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse spleen lysate 10µg
    Lane 2: ab243658 IP in Mouse spleen lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243658 in Mouse spleen lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 min

  • Flow Cytometry - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)
    Flow Cytometry - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

    Flow cytometric analysis of Mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/500 compared with a Rabbit monoclonal IgG (ab172730, Left) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)
    Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in some subsets of mouse splenocytes is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)
    Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in EL4 cells is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Anti-SLAMF6 antibody [EPR23122-130] (ab243658)
    Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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