Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

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Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23122-130] to SLAMF6
Suitable for: ICC/IF, Flow Cyt, IP
Reacts with: Mouse

Product name

Anti-SLAMF6 antibody [EPR23122-130]
See all SLAMF6 primary antibodies
Description

Rabbit monoclonal [EPR23122-130] to SLAMF6
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IP	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: EL4 and mouse splenocytes cells. Flow Cyt: Mouse splenocytes cells. IP: Mouse spleen lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23122-130
Isotype

IgG
Research areas

Immunoprecipitation - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

SLAMF6 was immunoprecipitated from 0.35 mg Mouse spleen lysate with ab243658 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243658 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: Mouse spleen lysate 10µg
Lane 2: ab243658 IP in Mouse spleen lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243658 in Mouse spleen lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 min
Flow Cytometry - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

Flow cytometric analysis of Mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/500 compared with a Rabbit monoclonal IgG (ab172730, Left) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor^® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in some subsets of mouse splenocytes is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Alexa Fluor^® 488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor^® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in EL4 cells is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Alexa Fluor^® 488 Goat anti-Rabbit secondary at 1/1000 dilution.
Anti-SLAMF6 antibody [EPR23122-130] (ab243658)

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