Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLAMF6 antibody [EPR22170] (ab224201)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22170] to SLAMF6
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SLAMF6 antibody [EPR22170]
    See all SLAMF6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22170] to SLAMF6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and Ramos whole cell lysates; Human lymph node and tonsil lysates. ICC/IF: Jurkat and Ramos cells. Flow Cyt: Ramos and human peripheral blood mononuclear cells. IP: Jurkat and Ramos whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22170

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] (ab224201)
    Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] (ab224201)

    Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling SLAMF6 with ab224201 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Western blot - Anti-SLAMF6 antibody [EPR22170] (ab224201)
    Western blot - Anti-SLAMF6 antibody [EPR22170] (ab224201)
    All lanes : Anti-SLAMF6 antibody [EPR22170] (ab224201) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : Human lymph node lysate
    Lane 4 : Human tonsil lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 37 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] (ab224201)
    Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] (ab224201)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (human Burkitt's lymphoma cell line) cells labeling SLAMF6 with ab224201 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in the Ramos cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] (ab224201)
    Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] (ab224201)

    SLAMF6 was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab224201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Ramos whole cell lysate 10 µg (Input).
    Lane 2: ab224201 IP in Ramos whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224201 in Ramos whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This blot was developed using a high sensitivity ECL substrate.

  • Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] (ab224201)
    Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] (ab224201)

    SLAMF6 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab224201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224201 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Jurkat whole cell lysate 10 µg (Input).
    Lane 2: ab224201 IP in Jurkat whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224201 in Jurkat whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This blot was developed using a high sensitivity ECL substrate.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] (ab224201)
    Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] (ab224201)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling SLAMF6 with ab224201 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in the Jurkat cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] (ab224201)
    Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] (ab224201)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling SLAMF6 with ab224201 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Anti-SLAMF6 antibody [EPR22170] (ab224201)
    Anti-SLAMF6 antibody [EPR22170] (ab224201)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-SLAMF6 antibody [EPR22170] (ab224201)

© 2021 Astana Biomed Group LLP. All rights reserved.