Human SLAM7/CS1 ELISA Kit (ab277429)
Key features and details
- Sensitivity: 0.83 ng/ml
- Range: 0.819 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SLAM7/CS1 ELISA Kit
See all SLAMF7/CS1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.83 ng/ml -
Range
0.819 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 118.8 94% - 139% Plasma 119.1 93% - 143% Cell culture media 102.4 89% - 114% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human SLAM7/CS1 ELISA Kit (ab277429) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SLAM7/CS1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human SLAM7/CS1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human SLAM7/CS1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human SLAM7/CS1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human SLAM7/CS1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated Anti-Human SLAM7/CS1 Antibody 2 vials Anti-Human SLAM7 CS1 coated Microplate 1 unit Human SLAM7/CS1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 does not mediate any activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro). SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. The isoform 3 is expressed at much lower level than isoform 1. There are three named isoforms. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Alternative names
- 19A
- 19A24 protein
- CD 319
see all -
Database links
- Entrez Gene: 57823 Human
- Omim: 606625 Human
- SwissProt: Q9NQ25 Human
- Unigene: 517265 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.