Anti-CD16 antibody [EPR20537] - BSA and Azide free (ab236765)
![Anti-CD16 antibody [EPR20537] - BSA and Azide free (ab236765)](https://www.abcam.com/ps/products/236/ab236765/Images/ab236765-313610-anti-cd16-antibody-epr-20537-immunofluorescence.jpg "Anti-CD16 antibody [EPR20537] - BSA and Azide free (ab236765)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20537] to CD16 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
Overview
-
Product name
Anti-CD16 antibody [EPR20537] - BSA and Azide free
See all CD16 primary antibodies -
Description
Rabbit monoclonal [EPR20537] to CD16 - BSA and Azide free -
Host species
Rabbit -
Specificity
We recommend ICC for human only. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: Jurkat cells.
-
General notes
Ab236765 is the carrier-free version of ab211151. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab236765 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20537 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-CD16 antibody [EPR20537] - BSA and Azide free (ab236765)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD16 with ab211151 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Jurkat cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211151).
-
![Anti-CD16 antibody [EPR20537] - BSA and Azide free (ab236765)](https://www.abcam.com/ps/products/236/ab236765/Images/ab236765-2-benefits-of-recombinant-antibodies.png)
Anti-CD16 antibody [EPR20537] - BSA and Azide free (ab236765)
