Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16784] to CD16 - C-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-CD16 antibody [EPR16784] - C-terminal
    See all CD16 primary antibodies

  • Description

    Rabbit monoclonal [EPR16784] to CD16 - C-terminal

  • Host species

    Rabbit

  • Specificity

    Rat species is recommended based on IHC result, we do not guarantee it for western blot.

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver, human thymus and rat liver tissues. WB: Human spleen tissue lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kupffer cell of rat liver tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)
    Western blot - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)
    Anti-CD16 antibody [EPR16784] - C-terminal (ab198507) at 1/1000 dilution + Human spleen tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 29 kDa
    Observed band size: 40-60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on macrophage of Human thymus tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kupffer cell of Human liver tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)
    Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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