Anti-GSTT2 antibody [EPR8136(2)] (ab176336)
![Anti-GSTT2 antibody [EPR8136(2)] (ab176336)](https://www.abcam.com/ps/products/176/ab176336/Images/ab176336-384512-anti-gstt2-antibody-epr81362-western-blot-hela-human-lung-a549.jpg "Anti-GSTT2 antibody [EPR8136(2)] (ab176336)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8136(2)] to GSTT2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-GSTT2 antibody [EPR8136(2)]
See all GSTT2 primary antibodies -
Description
Rabbit monoclonal [EPR8136(2)] to GSTT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human GSTT2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P0CG30 -
Positive control
- WB: Human prostate, mouse lung, and human lung tissue lysates, HeLa cell lysate.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as Glutathione S Transferase theta 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8136(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSTT2 antibody [EPR8136(2)] (ab176336)All lanes : Anti-GSTT2 antibody [EPR8136(2)] (ab176336) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GSTT2B knockout HeLa cell lysate
Lane 3 : Human lung tissue lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDaLanes 1-4: Merged signal (red and green). Green - ab176336 observed at 28 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab176336 Anti-GSTT2 antibody [EPR8136(2)] was shown to specifically react with GSTT2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264703 (knockout cell lysate ab258443) was used. Wild-type and GSTT2 knockout samples were subjected to SDS-PAGE. ab176336 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GSTT2 antibody [EPR8136(2)] (ab176336)](https://www.abcam.com/ps/products/176/ab176336/Images/ab176336-203586-WB6.jpg)
Western blot - Anti-GSTT2 antibody [EPR8136(2)] (ab176336)All lanes : Anti-GSTT2 antibody [EPR8136(2)] (ab176336) at 1/1000 dilution
Lane 1 : Human prostate lysate
Lane 2 : Mouse lung lysate
Lane 3 : Fetal lung lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa
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![Anti-GSTT2 antibody [EPR8136(2)] (ab176336)](https://www.abcam.com/ps/products/176/ab176336/Images/ab176336-3-benefits-of-recombinant-antibodies.png)
Anti-GSTT2 antibody [EPR8136(2)] (ab176336)
