Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP189] to CD16a - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD16a antibody [SP189] - BSA and Azide free
    See all CD16a primary antibodies

  • Description

    Rabbit monoclonal [SP189] to CD16a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung, liver, thymus, spleen, placenta, B-cell lymphoma, cervical squamous cell carcinoma, colon carcinoma, HK lymphoma, stomach carcinoma, and liver hepatocellular carcinoma tissue; Flow Cyt: Jurkat cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab243936 is the carrier-free version of ab227665. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243936 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CD16a with ab227665 at 1/100 dilution (1.47 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227665)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD16a with ab227665 at 1/100 dilution (1.47 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227665)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Flow Cytometry - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Flow Cytometry - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD16a with ab227665 at 1/100 (green) compared with a rabbit isotype control (blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human liver tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human lung tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human lung tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

  • Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
    Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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