Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Wednesday March 03, 2021

Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2866] to Tau (phospho S422)
Suitable for: WB, Dot blot, Agglutination
Reacts with: Human

Product name

Anti-Tau (phospho S422) antibody [EPR2866]
See all Tau primary antibodies
Description

Rabbit monoclonal [EPR2866] to Tau (phospho S422)
Host species

Rabbit
Specificity

ab79415 only detects Tau phosphorylated on serine 422.
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Tau aa 400-500 (C terminal). The exact sequence is proprietary.
Positive control
- WB: IMR-32 and Okadiac acid and calyculin A treated SH-SY5Y cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2866
Isotype

IgG
Research areas

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)

All lanes : Anti-Tau (phospho S422) antibody [EPR2866] (ab79415) at 1/1000 dilution

Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate
Lane 2 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate. The membrane was incubated with phosphatase.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)

Dot blot analysis of Tau (pS422) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS422) with ab79415 at a dilution of 1/1000. ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)

All lanes : Anti-Tau (phospho S422) antibody [EPR2866] (ab79415) at 1/20000 dilution

Lane 1 : SH-SY5Y cell lysates
Lane 2 : SH-SY5Y cell lysates treated with Okadiac acid and calyculin A

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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