Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2866] to Tau (phospho S422)
  • Suitable for: WB, Dot blot, Agglutination
  • Reacts with: Human

Overview

  • Product name

    Anti-Tau (phospho S422) antibody [EPR2866]
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR2866] to Tau (phospho S422)

  • Host species

    Rabbit

  • Specificity

    ab79415 only detects Tau phosphorylated on serine 422.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Tau aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: IMR-32 and Okadiac acid and calyculin A treated SH-SY5Y cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)
    Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)
    All lanes : Anti-Tau (phospho S422) antibody [EPR2866] (ab79415) at 1/1000 dilution

    Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate
    Lane 2 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate. The membrane was incubated with phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)
    Dot Blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)

    Dot blot analysis of Tau (pS422) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS422) with ab79415 at a dilution of 1/1000. ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)
    Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)
    All lanes : Anti-Tau (phospho S422) antibody [EPR2866] (ab79415) at 1/20000 dilution

    Lane 1 : SH-SY5Y cell lysates
    Lane 2 : SH-SY5Y cell lysates treated with Okadiac acid and calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 79 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?

  • Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)
    Anti-Tau (phospho S422) antibody [EPR2866] (ab79415)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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