Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2929Y] to Prealbumin - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free
    See all Prealbumin primary antibodies

  • Description

    Rabbit monoclonal [EP2929Y] to Prealbumin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Human heart lysate.

  • General notes

    ab185127 is the carrier-free version of ab75815 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab185127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)

    ab75815 at 1/250 dilution staining Prealbumin in human liver by Immunohistochemistry, Paraffin-embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75815).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)
    Immunocytochemistry - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)

    Clone EP2929Y (ab185127) has been successfully conjugated by Abcam. This image was generated using Anti-Prealbumin antibody [EP2929Y] (Alexa Fluor® 488). Please refer to ab199074 for protocol details.

    ab199074 staining Prealbumin in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199074 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)
    Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)

    This data was developed using ab75815, the same antibody clone in a different buffer formulation.
    Purified ab75815 at 1/50 dilution (2µg) immunoprecipitating Prealbumin in Human heart lysate.
    Lane 1 (input): Human heart lysate 10µg
    Lane 2 (+): ab75815 + Human heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75815 in Human heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 16 kDa

  • Flow Cytometry - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)
    Flow Cytometry - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)

    Flow Cytometry analysis of HepG2 cells labelling Prealbumin with ab75815 at a dilution of 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75815).

  • Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)
    Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (ab185127)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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