Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸

Order now and get it on Thursday March 04, 2021

Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2401Y] to Tau (phospho S199)
Suitable for: WB, Dot blot
Reacts with: Mouse, Human

Product name

Anti-Tau (phospho S199) antibody [EPR2401Y]
See all Tau primary antibodies
Description

Rabbit monoclonal [EPR2401Y] to Tau (phospho S199)
Host species

Rabbit
Specificity

ab81268 only detects Tau phosphorylated on Serine 199.
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic phospho peptide (phospho S199) corresponding to residues surrounding Serine 199 of Human Tau.
Positive control
- WB: Mouse cerebral cortex and human hippocampus tissue lysates, SH SY5Y cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2401Y
Isotype

IgG
Research areas

Western blot - Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268)

All lanes : Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268) at 1/1000 dilution

Lane 1 : Mouse cerebral cortex tissue lysate
Lane 2 : Mouse cerebral cortex tissue lysate. The membrane was incubated with phosphatase.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 79 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268)

All lanes : Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268) at 1/1000 dilution

Lane 1 : Human hippocampus tissue lysate
Lane 2 : Human hippocampus tissue lysate. The membrane was incubated with phosphatase.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

Predicted band size: 79 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking and dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268)

Dot blot analysis of Tau (pS199) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS199) with ab81268 at a dilution of 1/1000. ab97051 (peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Exposure time: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268)

All lanes : Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268) at 1/20000 dilution

Lane 1 : SH SY5Y cell lysate
Lane 2 : SH SY5Y cell lysate treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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