Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1884(2)] to Tau (phospho S214)
Suitable for: Dot blot, IHC-P, WB
Reacts with: Mouse, Human

Product name

Anti-Tau (phospho S214) antibody [EPR1884(2)]
See all Tau primary antibodies
Description

Rabbit monoclonal [EPR1884(2)] to Tau (phospho S214)
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

within Human Tau aa 200-300 (phospho S214). The exact sequence is proprietary.
Database link: P10636
Positive control
- WB:SH-SY5Y and C57 mouse cerebral cortex cell lysates. IHC: Human brain, normal spleen, normal kidney, cervical carcinoma and glioma tissues; Mouse brain tissue. Dot Blot:Tau (phospho S214) phospho peptide and Tau non-phospho peptide.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1884(2)
Isotype

IgG
Research areas

Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

All lanes : Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892) at 1/1000 dilution

Lane 1 : C57 mouse cerebral cortex whole cell lysates.
Lane 2 : C57 mouse cerebral cortex whole cell lysates. The membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 50-70 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling Tau (phospho S214) with ab170892 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

ab170892 showing +ve staining in Mouse brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

All lanes : Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892) at 1/1000 dilution

Lane 1 : SH-SY5Y cell lysates untreated
Lane 2 : SH-SY5Y cell lysates treated with Okadic acid + Calyculin A.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 78 kDa
Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

Dot blot analysis of Tau (phospho S214) phospho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labeling Tau (phospho S214) with ab170892 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Tau (phospho S214) with ab170892 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

ab170892 showing -ve staining in Human normal spleen tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

ab170892 showing -ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

ab170892 showing -ve staining in Human cervical carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

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