Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)
![Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)](https://www.abcam.com/ps/products/223/ab223192/Images/ab223192-319046-anti-tau-phospho-t181-antibody-epr22064-western-blot.jpg "Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22064] to Tau (phospho T181)
- Suitable for: WB, Dot blot, IP
- Reacts with: Human
Overview
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Product name
Anti-Tau (phospho T181) antibody [EPR22064]
See all Tau primary antibodies -
Description
Rabbit monoclonal [EPR22064] to Tau (phospho T181) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain lysate. Dot blot: Tau (phospho T181) peptide. IP: Human brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22064 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)All lanes : Anti-Tau (phospho T181) antibody [EPR22064] (ab223192) at 1/1000 dilution
Lane 1 : Human brain lysate
Lane 2 : Human brain lysate (WB membrane treated with alkaline phosphatase)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 46 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
WB membrane in Lane 2 was treated with alkaline phosphatase for 1h at 37°C.
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![Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)](https://www.abcam.com/ps/products/223/ab223192/Images/ab223192-319047-anti-tau-phospho-t181-antibody-epr22064-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)Tau (phospho T181 was immunoprecipitated from 0.35 mg of human brain lysate with ab223192 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223192 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human brain lysate 10 μg (Input).
Lane 2: ab223192 IP in Human brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223192 in Human brain lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 3 seconds. -
![Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)](https://www.abcam.com/ps/products/223/ab223192/Images/ab223192-319045-anti-tau-phospho-t181-antibody-epr22064-dot-blot.jpg)
Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)Dot blot analysis of Tau (phospho T181) labeled with ab223192 at 1/1000 dilution.
Lane 1: Tau (phospho T181) peptide.
Lane 2: Tau non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)](https://www.abcam.com/ps/products/223/ab223192/Images/ab223192-4-benefits-of-recombinant-antibodies.png)
Anti-Tau (phospho T181) antibody [EPR22064] (ab223192)
