Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)
![Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)](https://www.abcam.com/ps/products/210/ab210044/Images/ab210044-259600-ab210044FLOW1.jpg "Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 405 Rabbit monoclonal [EPR17526] to ERK1 + ERK2
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Overview
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Product name
Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526]
See all ERK1 + ERK2 primary antibodies -
Description
Alexa Fluor® 405 Rabbit monoclonal [EPR17526] to ERK1 + ERK2 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 405. Ex: 402nm, Em: 421nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P28482 -
Positive control
- Flow Cyt: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17526 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)
Overlay histogram showing A431 cells stained with ab210044 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22ºC and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22ºC. Cells were then incubated with the antibody (ab210044, 1/500 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.
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![Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)](https://www.abcam.com/ps/products/210/ab210044/Images/ab210044-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)
