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Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] - BSA and Azide free (ab242418)

Key features and details

  • Rabbit monoclonal [SP327] to ERK1 + ERK2 (phospho T202 + Y204) - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] - BSA and Azide free
    See all ERK1 + ERK2 primary antibodies

  • Description

    Rabbit monoclonal [SP327] to ERK1 + ERK2 (phospho T202 + Y204) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human ERK1 + ERK2 (phospho T202). The exact sequence is proprietary.
    Database link: P27361

  • Positive control

    • WB: A431 + EGF cell lysates. IHC-P: Human breast ductal carcinoma, bladder transitional cell carcinoma and breast tissues. Flow Cyt: A431 + EGF cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab242418 is the carrier-free version of ab223500. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242418 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] - BSA and Azide free (ab242418)
    Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] - BSA and Azide free (ab242418)

    Flow cytometric analysis of ab223500 in A431 (human epidermoid carcinoma cell line) cells + EGF (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] - BSA and Azide free (ab242418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] - BSA and Azide free (ab242418)

    Paraffin-embedded human breast ductal carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] - BSA and Azide free (ab242418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] - BSA and Azide free (ab242418)

    Paraffin-embedded human breast tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody [SP327] - BSA and Azide free (ab242418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody [SP327] - BSA and Azide free (ab242418)

    Paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223500).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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