HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)
![HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)](https://www.abcam.com/ps/products/209/ab209321/Images/ab209321-265735-anti-erk1-erk2-antibody-epr17526-hrp-western-blot.jpg "HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17526] to ERK1 + ERK2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-ERK1 + ERK2 antibody [EPR17526]
See all ERK1 + ERK2 primary antibodies -
Description
HRP Rabbit monoclonal [EPR17526] to ERK1 + ERK2 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P28482 -
Positive control
- WB: HeLa, HepG2, RAW 264.7, NIH 3T3, C6 and PC12 whole cell and Human Fetal Heart tissue lysates. Also ERK1 Recombinant Protein and ERK2 Recombinant Protein (data not shown).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17526 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)All lanes : HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : C6 (Rat glioma cell line) Whole Cell Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Heart (Human) Tissue Lysate - fetal normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43, 41 kDa
Observed band size: 39,41 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209321 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)](https://www.abcam.com/ps/products/209/ab209321/Images/ab209321-2-benefits-of-recombinant-antibodies.png)
HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)
