Anti-Tau (phospho S409) antibody (ab4861)
Key features and details
- Rabbit polyclonal to Tau (phospho S409)
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Tau (phospho S409) antibody
See all Tau primary antibodies -
Description
Rabbit polyclonal to Tau (phospho S409) -
Host species
Rabbit -
Specificity
Phosphorylation site-specific antibody selective for the phosphorylated form of human tau containing a phosphate on serine 409. -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
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Positive control
- Recombinant human Tau left untreated, or treated with PKA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at serine 409. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tau (phospho S409) antibody (ab4861)Recombinant human tau was (a) untreated, (b) phosphorylated in vitro by casein kinase II, and (c) phosphorylated in vitro by the catalytic subunit of protein kinase A. Protein was resolved by SDS-PAGE on a 4-20% Tris glycine gel and transferred to PVDF. Membranes were incubated with 1/1000 dilution of pan tau antibody or 0.5
µ g/mL anti-tau [pS409]. After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG or anti-mouse IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that the anti-tau pS409 antibody only recognizes recombinant tau protein phosphorylated by the catalytic subunit of protein kinase A, demonstrating the specificity of this antibody. Recombinant human tau was (a) untreated, (b) phosphorylated in vitro by casein kinase II, and (c) phosphorylated in vitro by the catalytic subunit of protein kinase A. Protein was resolved by SDS-PAGE on a 4-20% Tris glycine gel and transferred to P -
Western blot - Anti-Tau (phospho S409) antibody (ab4861)WB using ab4861. The peptide corresponding to Tau [pS409] blocks the antibody signal, thereby demonstrating the specificity of the antibody.
WB using ab4861. The peptide corresponding to Tau [pS409] blocks the antibody signal, thereby demonstrating the specificity of the antibody.
