Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR031F] to Indoleamine 2, 3-dioxygenase
Suitable for: WB, ICC, IHC-P
Reacts with: Human

Product name

Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F]
See all Indoleamine 2, 3-dioxygenase primary antibodies
Description

Rabbit monoclonal [BLR031F] to Indoleamine 2, 3-dioxygenase
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Indoleamine 2, 3-dioxygenase.
Database link: P14902
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR031F
Isotype

IgG
Research areas

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

Western blot analysis using ab243888 at 1/1000 dilution.
Lane 1: HeLa (- IFN-gamma) whole cell lysate (50 µg).
Lane 2: HeLa (+ IFN-gamma) whole cell lysate (50 µg).
Lane3: HEK-293T whole cell lysate (50 µg).
Lane 4: HDLM-2 whole cell lysate (50 µg).
Lane 5: HepG2 whole cell lysate (50 µg).
Lane 6: HEK-293T (expressing IDO2) whole cell lysate (50 µg).
Immunocytochemistry - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

Formalin-fixed, paraffin-embedded A549 (human lung carcinoma cell line) cells labeling Indoleamine 2,3-dioxygenase using ab243888 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. Untreated cells (left) and cells treated withhuman interferon-gamma (right) are shown. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for J130 using ab243888 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Indoleamine 2,3-dioxygenase using ab243888 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Indoleamine 2,3-dioxygenase using ab243888 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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